Publications by CPCRI Staff

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Browsing Publications by CPCRI Staff by Subject "2, 4-D"

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    Specific Nutritional Requirements of Coconut Calli (Cocos nucifera L) during Somatic Embryogenesis Induction
    (2007) Magnaval, C.; Buffard-Morel, J.; Beule, T.; Grosdemange, F.; Huet, C.; Blattes, A.; Verdeil, J.L.; Noirot, M.
    Coconut calli were cultivated on two somatic embryogenesis induction media (SEIMs), differing in their 2,4-D content. Gain in dry matter weight, composition of soluble sugars within calli, but also pH and contents of glucose and macroelements in media were analysed at 0, 15, 28, and 60 days of culture. Relationships between contents of endogenous sugars, on the one hand, and between contents of media macroelements, on the other hand, were analysed. Comparison was made with calli maintained on a control multiplication medium. Traits could be classified into 3 types of response with regard to condition of somatic embryogenesis induction (SEI condition). The first correspond to traits that were modified by the SHI condition and varying over time. Two phases were determined. During the first phase (T0-T15), soluble sugar contents within calli decreased over rime. The higher the 2,4-D content in SHIMs, the higher the sugar contents, Consumption of glucose and macroelements in media was negligible. However, strong relationships in the contents of chloride, nitrate, phosphate, and sulfatc were modified in the SF.I condition, During the second phase (TI5-1 60, growth became lower in the SEI condition. Requirements for glucose, nitrate and phosphate and acidification of media were higher. I he relationship, determined by changes in nitrate and phosphate (R>0.98), was modified by the SEI conditions, showing a preferential consumption for nitrate in this case. Endogenous sucrose content decreased to become lower in the SEI condition. The higher the 2,4-D content in SEIMs, the higher the requirements for media compounds, the higher the contents of sugars within calli, but the lower the growth. The second type of response corresponded to traits modified by the SEI condition, but constant over time. It concerned relationships between contents of some cations in the media. The third type of response corresponded to traits unchanges by the SEI condition and over time, It concerned the high relationship contents of endogenous glucose and fructose (R = 0,88), and between contents of chloride, ammonium, calcium, magnesium, and potassium.