Coconut-Molecular Biology

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    Stomatal conductance and photosynthesis
    (1982) Graham D. Farquhar; Thomas D. Sharkey
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    Identification of molecular markers associated with mite resistance in coconut (Cocos nucifera L.)
    (2007) Shalini, K.V.; Manjunatha, S.; Lebrun, P.; Berger, A.; Baudouin, L.; Ranganath, R.M.; Pirany, N.; Theertha Prasad, D.
    Coconut mite (Aceria guerreronis‘Keifer’) has become a major threat to Indian coconut (Coc¸cos nuciferaL.) cultivators and the processing industry. Chemical and biological control measures have proved to be costly, ineffective, and ecologically undesirable. Planting mite-resistant coconut cultivars is the most effective method of preventing yield loss and should form a major component of any integrated pest management stratagem. Coconut genotypes, and mite-resistant and -susceptible accessions were collected from different parts of South India. Thirty-two simple sequence repeat (SSR) and 7 RAPD primers were used for molecular analyses. In single-marker analysis, 9 SSR and 4 RAPD markers associated with mite resistance were identified. In stepwise multiple regression analysis of SSRs, a combination of 6 markers showed 100% association with mite infestation. Stepwise multiple regression analysis for RAPD data revealed that a combination of 3 markers accounted for 83.86% of mite resistance in the selected materials. Combined stepwise multiple regression analysis of RAPD and SSR data showed that a combination of 5 markers explained 100% of the association with mite resistance in coconut. Markers associated with mite resistance are important in coconut breeding programs and will facilitate the selection of mite-resistant plants at an early stage as well as mother plants for breeding programs.
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    Cross-Species Amplification of Coconut Microsatellite Markers in Rattans
    (2007) Nageswara Rao, M.; Ramesha, B.T.; Ravikanth, G.; Ganeshaiah, K.N.; Uma Shaanker, R.
    Cross-species amplification of microsatellite loci is considered to be a time-saving as well as a cost-effective approach for developing locus specific markers for new species. In an attempt to identify molecular markers to determine gene flow events and genetic differentiation of populations in rattans, microsatellite primer pairs developed for Cocos nucifera (Arecaceae) were examined for cross-species amplification in rattans. Of the six microsatellite primer pairs screened, two (CNZ34 and CN2A5) yielded good cross-species amplification across different rattan genera and species from South and Southeast Asia. Based on microsatellite-specific PCR amplification products, cluster analysis was performed. The dendrogram indicated a clear grouping of rattans based on their genera and species. The coconut locus specific primer pairs could be successfully used to construct molecular differentiation of rattan genera across different geographical locations.