Chemical analysis of Azospirillum lipopolysaccharides
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Date
1987
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Abstract
Lipopolysaccharides (LPS) were extracted by hot phenol-water from five strains each of Azospirillum lipoferum and Azospirillum brasilense. Rhamnose, glucose, glucosamine and 3-deoxy-D-mannooctulosonic acid were comon sugar constituents of all LPS preparations. 2-O-Me-fucose, 3-O-Me-fucose, 3-O-Me-rhamnose and 2-O-Me-galactose were found in LPSs of some A. brasilense strains. Fatty acid spectra from all LPSs studied were almost identical with predominance of 3-hydroxymyristic and 3-hydroxy-palmitic acids. 3-Hydroxypalmitic acid was the only amide-linked fatty acid. Lipopolysaccharides isolated from A. brasilense showed higher heterogeneity in sugar composition than those from A. lipoferum.
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Keywords
Azospirillum lipoferum, Azospirillum brasilense, Lipopolysaccharides, O-Methyl sugars, Fatty acids
Citation
Arch. Microbiol. (1987) 146 : 341-345