Identification and utilization of informative EST-SSR markers for genetic purity testing of coconut hybrids
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Date
2016-08
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Abstract
Coconut palms are categorized into two forms, viz., ‘talls’ and ‘dwarfs’ which are being utilized to produce hybrids through the
process of inter-varietal or intra-varietal crosses. Hybrid coconut seedlings are generally identified and selected based on
morphological traits by plant breeders, which is quite difficult and requires expertise. Even minor errors in identification may
adversely affect breeding programs in coconut, which is spread over many decades. In this study, we have utilized thirty EST-SSR
markers, derived from existing coconut leaf transcriptome data, for screening polymorphism between eighteen coconut parental
lines. The polymorphic primers capable of differentiating the parental palms were then utilized successfully for assessment of
purity of hybrids derived from these parents. Thus, the current study demonstrates the utility of EST-SSR markers in determining
the genetic purity of hybrids in coconut.
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Keywords
Coconut, hybrids, genetic purity, EST-SSR
Citation
Journal of Plantation Crops, 2016, 44(2): 77-84