Palm kernel agar: An alternative culture medium for rapid detection of aflatoxins in agricultural commodities

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dc.contributor.authorAtanda, O.O.
dc.contributor.authorAkpan, I.
dc.contributor.authorEnikuomehin, O.A.
dc.date.accessioned2014-05-13T09:48:27Z
dc.date.available2014-05-13T09:48:27Z
dc.date.issued2006
dc.description.abstractThe feasibility of using palm kernel agar (PKA) as an alternative culture medium to desiccated coconut agar (DCA), the conventional medium for the recovery of aflatoxigenic fungi from mixed cultures and the detection of aflatoxigenic fungi and direct visual determination of aflatoxins in agricultural commodities was assessed. The medium recovered aflatoxigenic fungi in 48 h from mixed cultures and agricultural commodities in 58 h as compared to 62 h obtained for the two treatments on desiccated coconut medium. Aflatoxigenic fungi were detected in all the agricultural commodities except for onions with maize having the highest value of 13.18% (w/w) followed by melon (10.97), yam flour (10.23) and groundnut (8.52) as against 11.48, 10.0, 6.92 and 8.52% (w/w) obtained for DCA. All aflatoxigenic strains produced a characteristic yellow pigmentation on a pink background and blue or blue green fluorescence of palm kernel agar Under long wave UV light (366nm) as against the white background of DCA, which often interferes with fluorescence with corresponding yield of aflatoxins. This shows that the medium is able to efficiently detect aflatoxin production through direct visual observation of fluorescence. Palm kernel agar (PKA) can therefore be routinely used as an alternative culture medium for screening aflatoxigenic fungi and direct visual determination of aflatoxins in agricultural commodities since it is faster and has a unique pink background for easy identification.en_US
dc.identifier.citationAfrican Journal of Biotechnology Vol. 5 (10), pp. 1029-1033, 16 May 2006en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1223
dc.language.isoenen_US
dc.subjectAflatoxinsen_US
dc.subjectaflatoxigenic mouldsen_US
dc.subjectmixed culturesen_US
dc.subjectand desiccated coconut agaren_US
dc.subjectpalm kernel agaren_US
dc.titlePalm kernel agar: An alternative culture medium for rapid detection of aflatoxins in agricultural commoditiesen_US
dc.typeArticleen_US

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