Genetic diversity in the coconut lethal yellowing disease phytoplasmas of East Africa

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dc.contributor.authorMpunami, A.A.
dc.contributor.authorTymon, A.
dc.contributor.authorJones, P.
dc.contributor.authorDickinson, M.J.
dc.date.accessioned2014-05-02T11:18:36Z
dc.date.available2014-05-02T11:18:36Z
dc.date.issued1999
dc.description.abstractDNA primers, based on the ribosomal sequences of lethal yellowing-type disease (LYD) phytoplasmas, were used to analyse genetic variation within the lethal yellowing-type diseases of coconut in East Africa. Samples were collected from palms in Kenya, Mozambique and high, medium and low disease incidence areas of Tanzania. The mollicutespecific primer pair P1 and P6 amplified a 1ยท5 kbp product from all diseased palms and no product from symptomless palms, indicating that phytoplasmas were associated with all of these diseases. However, the Rohde forward and Rohde reverse primers (a second rRNA primer pair designed to detect East African LYD-associated phytoplasmas) only amplified products from Tanzanian and Kenyan diseased palms and not from those of Mozambique. Conversely, primers Ghana 813 and AK-SR, designed for specific detection of coconut-associated phytoplasmas in West Africa, amplified products only from the Mozambique palms, indicating that the phytoplasma associated with LYD in Mozambique is more closely related to those fromWest Africa. This was supported by restriction enzyme digestion of PCR products. DNA sequencing of PCR products from phytoplasmas within Tanzania revealed no detectable differences in the rDNA sequences of isolates from high, medium and low incidence areas.en_US
dc.identifier.citationPlant Pathology (1999) 48, 109โ€“114en_US
dc.identifier.urihttp://hdl.handle.net/123456789/849
dc.language.isoenen_US
dc.subjectcoconuten_US
dc.subjectgenetic diversityen_US
dc.subjectlethal yellowsen_US
dc.subjectPCRen_US
dc.subjectphytoplasmaen_US
dc.subjectrDNAen_US
dc.titleGenetic diversity in the coconut lethal yellowing disease phytoplasmas of East Africaen_US
dc.typeArticleen_US

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