Studies on genetic transformation of Theobroma cacao L.: evaluation of different polyamines and antibiotics on somatic embryogenesis and the efficiency of uidA gene transfer by Agrobacterium tumefaciens

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dc.contributor.authorThiago E.R. Silva
dc.contributor.authorLuciana C. Cidade
dc.contributor.authorFatima C. Alvim
dc.contributor.authorJulio C.M. Cascardo
dc.contributor.authorMarcio G.C. Costa
dc.date.accessioned2014-04-17T11:05:27Z
dc.date.available2014-04-17T11:05:27Z
dc.date.issued2009
dc.description.abstractIn order to develop a more efficient genetic transformation system for cacao somatic embryos, the effects of polyamines and b-lactam antibiotics on somatic embryogenesis, hygromycin as selective agent, and different factors affecting uidA gene transfer have been evaluated. The polyamines putrescine, spermidine, and spermine significantly improved secondary somatic embryogenesis in cacao. Spermine at 1,000 lM provided the best responses, increasing 6.79 the percentage of embryogenic callus and 2.59 the average number of embryos per embryogenic callus. The b-lactam antibiotics timentin and meropenem, used for Agrobacterium tumefaciens counter-selection, had a nondetrimental effect on secondary somatic embryogenesis, depending on their concentration, whereas the commonly used b-lactam cefotaxime inhibited it, irrespective of the tested concentration. Hygromycin showed a strong inhibitory effect on secondary somatic embryogenesis of cacao, impairing completely the embryo production at 20 mg l-1. Following the criterion of GUS activity, the best conditions for T-DNA transfer into cotyledon explants from primary somatic embryos of cacao were a sonication of the explants for 100 s, a 20-min incubation period in Agrobacterium solution, an Agrobacterium concentration of 1.0 (OD600), and cocultivation of the explants on tobacco feeder layers. These findings will have important implications for studies on functional genomics of cacaoen_US
dc.identifier.citationPlant Cell Tiss Organ Cult (2009) 99:287–298en_US
dc.identifier.urihttp://hdl.handle.net/123456789/425
dc.language.isoenen_US
dc.subjectCocoaen_US
dc.subjectPutrescineen_US
dc.subjectSpermidineen_US
dc.subjectSpermineen_US
dc.subjectBeta-lactam antibioticsen_US
dc.subjectSAATen_US
dc.titleStudies on genetic transformation of Theobroma cacao L.: evaluation of different polyamines and antibiotics on somatic embryogenesis and the efficiency of uidA gene transfer by Agrobacterium tumefaciensen_US
dc.typeArticleen_US

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