Effects of Plant Growth Regulators on High Frequency Shoot Multiplication and Callus Regeneration of an Important Indian Medicinal Plant, Nirgundi (Vitex Negundo L.)
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Date
2006
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Abstract
A rapid shoot multiplication protocol was established for an important medicinal plant, Vitex negundo L., belonging to the family Verbenaceae, using Murashige and Skoog medium, achieved by shoot multiplication as well as callus regeneration. Shoot multiplication was induced by different concentrations of 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ), Benzyladenine and 6-furfuryl amino purine separately along with 10% (v/v) coconut water. Green organogenetic callus was obtained by the combined effect of 0.5–2.15mM TDZ and 1.7mM indole-3-acetic acid (IAA) along with 1% polyvinylpyrrolidone (PVP), and produced the maximum number of shoots when subcultured onto medium containing 2.7mM TDZ alone. Elongation of in vitro shoots was observed in MS medium containing 2.4 mM gibberellic acid and rooting was induced by the combined effect of 1.71mM IAA and 1.62mM a-naphthalene acetic acid.
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acclimatization, coconut water, multiple shoots, organogenesis, Verbenaceae, Vitex negundo L.
Citation
In Vitro Cell. Dev. Biol.—Plant 42:69–73, January–February 2006