Direct shoot regeneration from nodal explants of Sida cordifolia Linn
Loading...
Date
2007
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Amethod was developed to initiatemultiple shoots from mature nodal explants of Sida cordifolia Linn. High frequency of regeneration was achieved on Murashige and Skoog (MS) medium supplemented with 2.0 mg l−1 6- benzylaminopurine, 0.5 mg l−1 α-naphthalene acidic acid, 1.0 mg l−1 adenine sulfate, and 10% (v/v) coconut milk. Multiple shoots were initiated within 21 d and the above media was capable of inducing the formation of more than 20 shoots from each explant. Regenerated shoots were successfully rooted on half-strength MS medium supplemented with 2.0 mg l−1 indole-3-butyric acid and 3% (w/v) sucrose. Rooted plantlets were established in soil. The regenerated plantlets showed no morphological differences from the parent material. This protocol could be useful for germplasm conservation, cultivation, and genetic improvement of S. cordifolia.
Description
Keywords
Adenine sulfate, Coconut milk, Nodal explants, Regeneration, Sida cordifolia
Citation
In Vitro Cell.Dev.Biol.—Plant (2007) 43:436–441