Cryopreservation by encapsulation–dehydration of plumules of coconut (Cocos nucifera L.)
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Date
2008
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Abstract
This study describes the use of an encapsulation-dehydration cryopreservation technique
on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In
order to establish a reliable cryopreservation process for plumules, several different key
factors were tested: pretreatment duration, sugar concentration, dehydration period and
freezing. In parallel, histological studies were performed to describe the structural changes of
tissues and plumule cells subjected to dehydration and freezing. A good survival level of
around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a
maximum of 20% which was achieved using sucrose treatment. In this paper we report for the
first time the regeneration of leafy shoots from coconut plumules after cryopreservation.
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Keywords
Cocos nucifera L., cryopreservation, encapsulation-dehydration, histology, plumule, sucrose
Citation
CryoLetters 29(4), 339-350 (2008)