Cryopreservation by encapsulation–dehydration of plumules of coconut (Cocos nucifera L.)
| dc.contributor.author | Oulo N’Nan | |
| dc.contributor.author | Valérie Hocher | |
| dc.contributor.author | Jean-Luc Verdeil | |
| dc.contributor.author | Jean-Louis Konan | |
| dc.contributor.author | Koffi Ballo | |
| dc.contributor.author | Fanja Mondeil | |
| dc.contributor.author | Bernard Malaurie | |
| dc.date.accessioned | 2017-01-19T10:04:37Z | |
| dc.date.available | 2017-01-19T10:04:37Z | |
| dc.date.issued | 2008 | |
| dc.description.abstract | This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20% which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation. | en_US |
| dc.identifier.citation | CryoLetters 29(4), 339-350 (2008) | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/6843 | |
| dc.language.iso | en | en_US |
| dc.subject | Cocos nucifera L. | en_US |
| dc.subject | cryopreservation | en_US |
| dc.subject | encapsulation-dehydration | en_US |
| dc.subject | histology | en_US |
| dc.subject | plumule | en_US |
| dc.subject | sucrose | en_US |
| dc.title | Cryopreservation by encapsulation–dehydration of plumules of coconut (Cocos nucifera L.) | en_US |
| dc.type | Article | en_US |