In vitro plant regeneration from immature cotyledon explants of macadamia (Macadamia tetraphylla L. Johnson)

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dc.contributor.authorRichard M.S. Mulwa
dc.contributor.authorPrem L. Bhalla
dc.date.accessioned2014-05-07T06:52:41Z
dc.date.available2014-05-07T06:52:41Z
dc.date.issued2006
dc.description.abstractThe macadamia tree, an Australian native, is highly valued for its nuts. Macadamia improvement programs so far have relied on conventional breeding and selection. The production of improved cultivars required to meet future demands could be accelerated by the application of modern biotechnological techniques, but this requires an efficient and reproducible regeneration system that has not yet been established for macadamia. We report here shoot regeneration from immature cotyledon explants of macadamia. Adventitious buds were induced on the cotyledon explants from fruits collected at 140 and 190 days after full bloom (DAFB) on MS medium supplemented with either 10 or 15 μM TDZ. The addition of 2% coconut milk (CM) to 10 μM TDZ containing media resulted in enhanced adventitious bud induction from 190 DAFB explants. Further shoot development from the induced buds was depressed in media containing TDZ + CM; the addition of 0.001 μM IAA to this combination doubled shoot development, from 1.9–3.9 shoots per explant. The transfer of bud clumps to media supplemented with 8.8 μM BA alone or in combination with either 0.14 μM GA3 or 0.001 μM IAA significantly increased shoot production from the previously induced explants by 1.5–2 times of that observed in TDZ + CM medium. Histological examinations revealed that shoot regeneration was primarily by organogenesis originating from cells on or just below the cut surfaces of explants.en_US
dc.identifier.citationPlant Cell Rep (2006) 25: 1281–1286en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1020
dc.language.isoenen_US
dc.subjectMacadamiaen_US
dc.subjectSomatic embryogenesisen_US
dc.subjectOrganogenesisen_US
dc.subjectHistologyen_US
dc.titleIn vitro plant regeneration from immature cotyledon explants of macadamia (Macadamia tetraphylla L. Johnson)en_US
dc.typeArticleen_US

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