Plantlet regeneration via direct and indirect somatic embryogenesis from inflorescence explants of arecanut palms (Areca catechu L.)

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dc.contributor.authorRadha, E.
dc.contributor.authorAnitha Karun
dc.contributor.authorAnanda, K.S.
dc.contributor.authorKumaran, P.M.
dc.date.accessioned2014-07-16T05:44:11Z
dc.date.available2014-07-16T05:44:11Z
dc.date.issued2006
dc.description.abstractPlantlet regeneration from inflorescence explants of yellow leaf disease (YLD) resistant arecanut cultivar south kanara local was achieved by both direct and indirect somatic embryogenesis. This is the first report of direct somatic embryogenesis from inflorescence explants of arecanut. A total of 10 inflorescences were extracted from healthy palms of YLD· hot spot areas from Sullia in Dakshina Kannada during 2005 and 2006. Out of these, direct somatic embryogenesis was obtained from six inflorescences, which is ideal because it allows the production of plants without a callus phase leading to somaclonal variation and hence useful for efficient genetic transformation. The vigour ofdirectly differentiated somatic embryos was much higher than indirect somatic embryo development. This was achieved in Y3 basal medium supplemented with two levels of picloram (1oollM, 200IlM). Induction of embryogenic potency and formation of somatic embryos were noticed when the cultures were passed from higher to lower concentration of picloram and then to a hormone free medium. Somatic embryos proliferated rapidly in subsequent cultures. Maturation of somatic embryos and germination occurred in Vz MS medium supplemented with Imgll BA and subsequent plantlet development was achieved by transferring them to same basal medium supplemented with 5 mg/l BA and O.5mgll IBA, followed by lOmgll BA, lmgll IBA and Img/l NAA. Anatomical studies were conducted at various developmental stages of both direct and indirect somatic embryogenesis. Histological studies revealed that somatic embryos arose indirectly from a single callus cell whereas the origin of direct somatic embryos was multicellular, without a callus phaseen_US
dc.identifier.citationJournal of Plantation Crops, 2006, 34(3) : 540-545en_US
dc.identifier.urihttp://hdl.handle.net/123456789/4147
dc.language.isoenen_US
dc.subjectArecanuten_US
dc.subjectYellow Leaf Diseaseen_US
dc.subjectsomatic embryogenesisen_US
dc.subjectin vitro multiplicationen_US
dc.subjectpicloramen_US
dc.titlePlantlet regeneration via direct and indirect somatic embryogenesis from inflorescence explants of arecanut palms (Areca catechu L.)en_US
dc.typeArticleen_US

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