Cryopreservation of coconut (Cocos nucifera L.) zygotic embryos after pre-growth desiccation

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dc.contributor.authorSajini, K.K.
dc.contributor.authorAnitha Karun
dc.contributor.authorKumaran, P.M.
dc.date.accessioned2014-06-06T07:16:29Z
dc.date.available2014-06-06T07:16:29Z
dc.date.issued2006
dc.description.abstractCoconut, being a recalcitrant species with large nuts, cryopreservation is the only option for long-term conservation of coconut . genetic resources, which can provide a viable backup to field gene banks. A simple cryopreservation technique involving pregrowth desiccation using sucrose was standardized for coconut zygotic embryos. Zygotic embryos extracted from WCT variety ofc!?conut were pre-grown in I M, 2M and 3M concentrations of sucrose and thereafter subjected to liquid nitrogen exposure for 24 hours. The results showed that 1M sucrose pre-treatment was not sufficient for dehydration as it resulted in death of the embryos after cryogenic treatment. At 2M and 3M sucrose concentrations, the moisture content of the embryos was reduced to 30% and 27% with the corresponding final recovery of plantlets after cryopreservation of 20.8% and 29%, respectively.en_US
dc.identifier.citationJournal of Plantation Crops 2006, 34(3) : 576-581en_US
dc.identifier.urihttp://hdl.handle.net/123456789/2481
dc.language.isoenen_US
dc.subjectCoconut zygotic embryosen_US
dc.subjectsucroseen_US
dc.subjectpre-growth desiccationen_US
dc.subjectcryogenic storageen_US
dc.titleCryopreservation of coconut (Cocos nucifera L.) zygotic embryos after pre-growth desiccationen_US
dc.typeArticleen_US

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