Identification and structural insights of three novel antimicrobial peptides isolated from green coconut water

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dc.contributor.authorSanti M. Mandal
dc.contributor.authorSatyahari Dey
dc.contributor.authorMahitosh Mandal
dc.contributor.authorSiddik Sarkar
dc.contributor.authorSimone Maria-Neto
dc.contributor.authorOctavio L. Franco
dc.date.accessioned2014-05-03T09:56:59Z
dc.date.available2014-05-03T09:56:59Z
dc.date.issued2009
dc.description.abstractInfections caused by pathogenic bacteria could cause an expressive negative impact on human health. A significant enhance in resistance to commercial antibiotics has been observed in all kinds of pathogenic bacteria. In order to find novel approaches to control such common infections, a wide number of defense peptides with bactericidal properties have been characterized. In this report, three peptides lower than 3 kDa were purified and identified from green coconut (Cocos nucifera L.) water by using reversed phasehigh performance liquid chromatography (HPLC), showing molecular masses of 858 Da, 1249 Da and 950 Da. First one, named Cn-AMP1, was extremely efficient against both Gram-positive and Gramnegative bacteria, being MICs calculated for three peptides. All complete sequences were determined by MALDI-ToF analysis showing no identity in databanks. Moreover, peptide net charge and hydrophobicity of each peptide was in silico evaluated. Finally molecular modeling and dynamics were also applied generating peptides three-dimensional structures, indicating a better explanation to probable mechanisms of action. Cn-AMPs here reported show remarkable potential to contribute in the development of novel antibiotics from natural sources.en_US
dc.identifier.citationPeptides 30 (2009) 633–637en_US
dc.identifier.urihttp://hdl.handle.net/123456789/921
dc.language.isoenen_US
dc.subjectAntibacterial activityen_US
dc.subjectGreen coconut wateren_US
dc.subjectAntimicrobial peptidesen_US
dc.subjectMass spectrometryen_US
dc.subjectCocos nuciferaen_US
dc.titleIdentification and structural insights of three novel antimicrobial peptides isolated from green coconut wateren_US
dc.typeArticleen_US

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