Modified protocol of ELISA for rapid detection of coconut root (wilt) disease

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dc.contributor.authorSasikala, M.
dc.contributor.authorRajeev, G.
dc.contributor.authorPrakash, V.R.
dc.contributor.authorAmith, S.
dc.date.accessioned2014-07-05T09:00:01Z
dc.date.available2014-07-05T09:00:01Z
dc.date.issued2010
dc.description.abstractRoot (wilt) disease of coconut is a phytoplasmal malady causing heavy economic loss to the coconut farmers in Kerala and Tamil Nadu. The disease is generally recognized based on foliar symptoms. But the development of foliar symptoms in coconut palms is very slow and there is a time lag between infection and symptom expression. To identify such latent infected palms, Direct Antigen Coated (DAC) indirect ELISA had been standardized earlier. But this ELISA system was not rapid. Though subsequently it was refined, 24 h was required for the completion of the test. Hence, a modified protocol was developed using leaf disc as test antigen. The modified protocol is simple, rapid and results could be obtained within 7 h. The test yielded very high sensitivity of 98 % with respect to visual observations. The modified protocol is being used for the routine screening of coconut palms for selection of disease-free mother palms for developing root (wilt) resistant/tolerant varieties. Besides this, the test is being used for confirmation of root (wilt) disease in coconut palms seen in mildly disease affected and disease outbreaks in new areas.en_US
dc.identifier.citationJournal of Plantation Crops. 2010.38 (1): 16-19en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3713
dc.language.isoenen_US
dc.subjectCoconuten_US
dc.subjectELISAen_US
dc.subjecthorse radish peroxidaseen_US
dc.subjectleaf discen_US
dc.subjectPhytoplasmaen_US
dc.subjectroot (wilt) diseaseen_US
dc.titleModified protocol of ELISA for rapid detection of coconut root (wilt) diseaseen_US
dc.typeArticleen_US

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