Plant regeneration through somatic embryogenesis from zygotic embryo-derived callus of areca catechu l. (arecaceae)
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Date
2003
Journal Title
Journal ISSN
Volume Title
Publisher
Society for In Vitro Biology
Abstract
An in vitro culture procedure was established for somatic embryogenesis and plant regeneration from callus cultures of the important palm ‘betel nut’ (Areca catechu L.). Segments of zygotic embryos of Areca catechu L. were cultured on Murashige and Skoog basal medium supplemented with dicamba (9.05, 18.1, 27.15, and 36.2mM). After 7–8 wk in darkness, wounded regions of explants formed callus with yellow, soft, glutinous structures. Proliferation and maintenance of callus was on the same dicamba-containing medium. With regular subculture every 8 wk, the callus showed pale yellow, compact and nodular structures. During subculture, somatic embryos were formed spontaneously from nodular callus tissues within 2–4 mo. The embryos developed into plantlets after 10 wk of culture on basal medium free of plant growth regulators. After subculturing every month for 3 mo., the plantlets were transferred to containers for acclimatization in the greenhouse. The survival rate was 24%.
Description
Keywords
betel nut, palm, somatic embryo
Citation
In Vitro Cell. Dev. Biol.—Plant 39:34–36, January–February 2003