Plant regeneration through somatic embryogenesis from zygotic embryo-derived callus of areca catechu l. (arecaceae)

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dc.contributor.authorHsiang-Chih Wang
dc.contributor.authorShieh-Ping Wu
dc.contributor.authorJen-Tsung Chen
dc.contributor.authorMei-Chun Lin
dc.contributor.authorWei-Chin Chang
dc.date.accessioned2014-04-02T11:25:21Z
dc.date.available2014-04-02T11:25:21Z
dc.date.issued2003
dc.description.abstractAn in vitro culture procedure was established for somatic embryogenesis and plant regeneration from callus cultures of the important palm ‘betel nut’ (Areca catechu L.). Segments of zygotic embryos of Areca catechu L. were cultured on Murashige and Skoog basal medium supplemented with dicamba (9.05, 18.1, 27.15, and 36.2mM). After 7–8 wk in darkness, wounded regions of explants formed callus with yellow, soft, glutinous structures. Proliferation and maintenance of callus was on the same dicamba-containing medium. With regular subculture every 8 wk, the callus showed pale yellow, compact and nodular structures. During subculture, somatic embryos were formed spontaneously from nodular callus tissues within 2–4 mo. The embryos developed into plantlets after 10 wk of culture on basal medium free of plant growth regulators. After subculturing every month for 3 mo., the plantlets were transferred to containers for acclimatization in the greenhouse. The survival rate was 24%.en_US
dc.identifier.citationIn Vitro Cell. Dev. Biol.—Plant 39:34–36, January–February 2003en_US
dc.identifier.urihttp://hdl.handle.net/123456789/189
dc.language.isoenen_US
dc.publisherSociety for In Vitro Biologyen_US
dc.subjectbetel nuten_US
dc.subjectpalmen_US
dc.subjectsomatic embryoen_US
dc.titlePlant regeneration through somatic embryogenesis from zygotic embryo-derived callus of areca catechu l. (arecaceae)en_US
dc.typeArticleen_US

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