Effect of growth regulators on microspore embryogenesis in coconut anthers

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dc.contributor.authorPerera, P.I.P.
dc.contributor.authorYakandawala, D.M.D.
dc.contributor.authorHocher, V.
dc.contributor.authorVerdeil, J.L.
dc.contributor.authorWeerakoon, L.K.
dc.date.accessioned2014-04-28T05:09:44Z
dc.date.available2014-04-28T05:09:44Z
dc.date.issued2009
dc.description.abstractThe effect of growth regulators on induction of androgenesis in coconut was investigated using seven different growth regulators at various concentrations and combinations. Three auxins (1-naphthalene acetic acid— NAA, indoleacetic acid—IAA, picloram) and three cytokinins (2-isopentyl adenine-2-iP, kinetin, zeatin) were tested either alone or in combination with 2,4-dichlorophenoxyacetic acid (2,4-D), using modified Eeuwens Y3 liquid medium as the basal medium. Among the tested auxins, 100 lM NAA in combination with 100 lM 2,4-D enhanced the production of calli/embryos (123) whereas IAA and picloram showed negative and detrimental effects, respectively, for androgenesis induction over 100 lM 2,4- D alone. Kinetin and 2-iP enhanced the production of calli/ embryos when 100 lM 2,4-D was present in the culture medium. Both cytokinins at 10 lM yielded the highest frequencies of embryos (113 and 93, respectively) whereas zeatin (1 or 2.5 lM) had no impact on microspore embryogenesis. When calli/embryos (produced from different treatments in different experiments) were subcultured in somatic embryo induction medium (Y3 medium containing 66 lM 2,4-D), followed by maturation medium (Y3 medium without growth regulators) and germination medium (Y3 medium containing 5 lM-6-benzyladenine— BA and 0.35 lM gibberellic acid—GA3), plantlets were regenerated at low frequencies (in most treatments ranging from 0% to 7%).en_US
dc.identifier.citationPlant Cell Tiss Organ Cult (2009) 96:171–180en_US
dc.identifier.urihttp://hdl.handle.net/123456789/658
dc.language.isoenen_US
dc.subjectAndrogenesisen_US
dc.subjectAnther cultureen_US
dc.subjectAuxinen_US
dc.subjectCytokininen_US
dc.subjectCallusen_US
dc.subjectCocos nuciferaen_US
dc.subjectEmbryoen_US
dc.titleEffect of growth regulators on microspore embryogenesis in coconut anthersen_US
dc.typeArticleen_US

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