Development of a RAPD-derived SCAR marker associated with tall-type palm trait in coconut

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dc.contributor.authorRajesh, M.K.
dc.contributor.authorJerard, B.A.
dc.contributor.authorPreethi, P.
dc.contributor.authorRegi Jacob Thomas
dc.contributor.authorFayas, T.P.
dc.contributor.authorRachana, K.E.
dc.contributor.authorAnitha Karun
dc.date.accessioned2014-06-07T10:06:03Z
dc.date.available2014-06-07T10:06:03Z
dc.date.issued2013
dc.description.abstractCoconut palms are classified into two major types, viz. talls and dwarfs, based on plant stature. The selection of coconut seedlings in the nursery, presently relying solely on morphological markers, often results in selection of out-crossed seedlings. Hence, identification of molecular markers for distinguishing tall/dwarf character at an early stage of growth assumes importance. In the present investigation, a RAPD marker for tall-type palm trait was identified using a pooled DNA approach. Screening of tall and dwarf palm bulked DNA with 200 decamer primers revealed a primer OPA09, producing a unique band of around 260 bp exclusively in tall accessions. The primer was used for screening and validation in individual tall and dwarf coconut accessions representing different geographic regions. The band was present in all the tall accessions, but absent in the dwarfs. Furthermore, sequence characterized amplified region (SCAR) primers were designed from the unique RAPD amplicon. The primers produced a specific 260-bp amplicon in tall accessions, but not in dwarf accessions. The SCAR marker was utilized in assessing the purity of hybrid seedlings of D×T (Dwarf×Tall) cross. The results pave the way for ensuring genuineness/quality of hybrid seedlings of coconut.en_US
dc.identifier.citationScientia Horticulturae 150(4):312–316: 2013en_US
dc.identifier.urihttp://hdl.handle.net/123456789/2554
dc.language.isoenen_US
dc.subjectCoconuten_US
dc.subjectRAPDen_US
dc.subjectPlant type traiten_US
dc.subjectSCARen_US
dc.titleDevelopment of a RAPD-derived SCAR marker associated with tall-type palm trait in coconuten_US
dc.typeArticleen_US

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