In situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease

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dc.contributor.authorIvan Cordova
dc.contributor.authorPhil Jones
dc.contributor.authorNigel A. Harrison
dc.contributor.authorCarlos Oropeza
dc.date.accessioned2014-05-06T06:17:49Z
dc.date.available2014-05-06T06:17:49Z
dc.date.issued2003
dc.description.abstractDNA of the lethal yellowing (LY) phytoplasma was detected in 13 of 72 embryos from fruits of four diseased Atlantic tall coconut palms by polymerase chain reaction (PCR) assays employing phytoplasma universal rRNA primer pair P1/P7, nested LY group-specific rRNA primer pair 503f/LY16Sr or LY phytoplasma-specific nonribosomal primer pair LYF1/R1. Phytoplasma distribution in sectioned tissues from six PCR positive embryos was determined by in situ PCR and digoxigenin-11-deoxy-UTP (Dig) labelling of amplification products. Dig-labeled DNA products detected by colourimetric assay were clearly evident on sections from the same three embryos investigated in detail by in situ PCRs employing primer pairs P1/P7 or LYF1/R1. Deposition of blue-green stain on sections as a result of each assay was restricted to areas of the embryos corresponding to the plumule and cells ensheathing it. By comparison, similarly treated embryo sections derived from fruits of a symptomless Atlantic tall coconut palm were consistently devoid of any stain. Presence of phytoplasma DNA in embryo tissues suggests the possible potential for seed transmission which remains to be demonstrated.en_US
dc.identifier.citationMolecular Plant Pathology (2003) 4 ( 2 ) pp 99–108en_US
dc.identifier.urihttp://hdl.handle.net/123456789/972
dc.language.isoenen_US
dc.titleIn situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing diseaseen_US
dc.typeArticleen_US

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