Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao
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Date
2008
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Abstract
The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium. Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo induction in ED medium was genotype-specifi c. Embryogenic callus formations increased in micropore tape-sealed Petri dishes, irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.
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Keywords
biotechnology, cacao, gas exchange, micropropagation, morphogenesis
Citation
Pesq. agropec. bras., Brasília, v.43, n.10, p.1433-1436, out. 2008