Regeneration of somatic embryos in Theobroma cacao L. in temporary immersion bioreactor and analyses of free amino acids in different tissues

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dc.contributor.authorKatja Saare-Surminski
dc.contributor.authorNicolas Niemenak
dc.contributor.authorDenis Omokolo Ndoumou
dc.contributor.authorChristina Rohsius
dc.contributor.authorReinhard Lieberei
dc.date.accessioned2014-04-17T07:25:10Z
dc.date.available2014-04-17T07:25:10Z
dc.date.issued2008
dc.description.abstractThe present study aimed at developing temporary immersion bioreactor techniques for multiplication of cacao somatic embryos. Temporary Immersion System (TIS), i.e. flooding of plant tissue at regular time intervals provides an efficient way to propagate plants. Somatic embryos were regenerated in twin flask bioreactors. The TIS proved to be suitable for mass regeneration of somatic embryos and for their subsequent direct sowing. The number of embryos after 3 months of culture was significantly higher in TIS cultures than in the solid medium variant. TIS also improved embryo development regarding the conversion to torpedo shaped forms. Matured embryos derived from TIS and pre-treated with 6% sucrose were converted into plants after direct sowing. Additionally to the influence of culture conditions on the development of somatic embryogenesis the content and composition of free amino acids were analysed. The content of free amino acids in somatic embryos rose as immersion frequency increased. The endogenous free GABA content in embryogenic callus was significantly higher than in non-embryogenic callus.en_US
dc.identifier.citationPlant Cell Rep (2008) 27:667–676en_US
dc.identifier.urihttp://hdl.handle.net/123456789/411
dc.language.isoenen_US
dc.subjectAmino acidsen_US
dc.subjectCacaoen_US
dc.subjectSomatic embryo developmenten_US
dc.subjectTwin flask bioreactoren_US
dc.subjectTISen_US
dc.titleRegeneration of somatic embryos in Theobroma cacao L. in temporary immersion bioreactor and analyses of free amino acids in different tissuesen_US
dc.typeArticleen_US

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