Theory and Practice of Rapid Liquid Chromatography at Moderate Pressures Using Water as Eluent
| dc.contributor.author | Michael R. Ladisch | |
| dc.contributor.author | George T. Tsao | |
| dc.date.accessioned | 2014-09-20T09:37:21Z | |
| dc.date.available | 2014-09-20T09:37:21Z | |
| dc.date.issued | 1978 | |
| dc.description.abstract | The rapid separation of oligo- and monosaccharides on long, narrow columns packed with easily compressed, 4% cross-linked, cation-exchange resin adds a new dimension to the chromatography of carbohydrates on gel type supports: speedy analysis at low pressure. The discovery of a pressure-critical diameter effect in the packing of Aminex 50W-X4 (Ca2+) makes it possible to pack 60-cm long columns capable of separating malto- and cellodextrins in 12-15 min using water as the sole eluent. The surprising phenomena of a 50-fold increase in pressure due to an increase in column diameter from 6 to 8 mm is reported and reasons for this effect are explained. Equally noteworthy is the stability of the 6-mm columns. One column described in this report has been in continuous operation for over 2600 h. The application of low-pressure liquid chromatography to enzyme kinetics as well as to separation of oligo-and monosaccharides is also discussed. | en_US |
| dc.identifier.citation | Journal of Chromatography, 166(1978) 85-100 | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/5923 | |
| dc.language.iso | en | en_US |
| dc.title | Theory and Practice of Rapid Liquid Chromatography at Moderate Pressures Using Water as Eluent | en_US |
| dc.type | Article | en_US |