Developmental changes in carboxylase activities in in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings

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dc.contributor.authorKarine Triques
dc.contributor.authorAlain Rival
dc.contributor.authorThierry Beule
dc.contributor.authorStephane Dussert
dc.contributor.authorValerie Hocher
dc.contributor.authorJean Luc Verdeil
dc.contributor.authorSerge Hamon
dc.date.accessioned2014-04-26T04:43:12Z
dc.date.available2014-04-26T04:43:12Z
dc.date.issued1997
dc.description.abstractPhosphoenolpyruvate Carboxylase (PEPC; EC: 4.1.1.31) and Ribulose 1,5-bisphosphate Carboxylase / Oxygenase (RubisCO; EC: 4.1.1.39) enzyme specific activities were measured during the in vitro development of coconut (Cocos nucifera L.) zygotic mature embryos into plantlets and compared with those of palms produced by conventional seed germination. At the time of initiation of germination, high PEPC and low RubisCO activities were measured in both cultured and conventionally germinated embryos, thus indicating an anaplerotic CO2 fixation. During both in vitro and in planta development, RubisCO progressively took over and became the main route for inorganic carbon fixation. The in vitro-grown coconut plantlets showed a faster decrease in their PEPC:RubisCO ratio than the seedlings, suggesting that an earlier transition from a heterotrophic to an autotrophic mode of carbon fixation takes place in the in vitro-derived material. Just before acclimatization, the RubisCO activity in in vitro-derived plantlets (2.83 mol CO2h-1mg-1 TSP) was lower than that in seedlings (6.98 mol CO2h-1mg-1 TSP) of the same age. Nevertheless, after acclimatization, RubisCO activities were comparable in both in vitro and in planta germinated materialen_US
dc.identifier.citationPlant Cell, Tissue and Organ Culture 49: 227–231, 1997.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/589
dc.language.isoenen_US
dc.subjectcarbon metabolismen_US
dc.subjectCO2 fixationen_US
dc.subjectembryo cultureen_US
dc.subjectPEPCen_US
dc.subjectphotosynthesisen_US
dc.subjectRubisCOen_US
dc.titleDevelopmental changes in carboxylase activities in in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlingsen_US
dc.typeArticleen_US

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