Assessing the genetic diversity in the International Cocoa Genebank Trinidad (ICG,T) using isozyme electrophoresis and RAPD
Loading...
Date
2005
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Random amplified polymorphic DNA (RAPD) and isozyme electrophoresis (IE) techniques were used to estimate the level of genetic diversity in a sample of cacao germplasm existing at the International Cocoa Genebank, Trinidad. Twenty-six cocoa populations represented by 459 cocoa genotypes were analysed using IE and 22 populations represented by 353 cocoa genotypes were analysed using RAPD. Despite few differences in the classification of the populations, both techniques revealed three major groups: the indigenous trees, the cultivated Trinitario and the cultivated trees from Ecuador. Two-thirds of the partitioned diversity were found within populations and one-third between the populations, with both techniques
Description
Keywords
Genetic diversity, Isozyme Electrophoresis, RAPD, Theobroma cacao L.
Citation
Genetic Resources and Crop Evolution (2005) 52: 1111–1120