Inter Simple Sequence Repeat (ISSR) markers for identification of germplasm and diversity analysis in coconut (Cocos nucifera L.)

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dc.contributor.authorManimekalai, R.
dc.date.accessioned2014-06-30T10:09:12Z
dc.date.available2014-06-30T10:09:12Z
dc.date.issued2012
dc.description.abstractInter Simple Sequence Repeat (ISSR) is a powerful molecular marker technique used for variety of purposes like genetic diversity analysis, phylogeny information, germplasm identification, gene tagging etc. In the present paper, 33 coconut (Cocos nucifera L.) germplasm representing different geographical regions have been fingerprinted with highly polymorphic ISSR markers. Ten polymorphic primers targeting the microsatellite regions were used to amplify the coconut germplasms. Ten primer produced 104 polymorphic markers with 87 percent polymorphism. Cluster analysis was carried out among 33 accessions by UPGMA using the software POPGENE ver.1.32. The dendrogram revealed five clusters. Based on the fingerprint data germplasm specific markers were identified using the primers UBC 810, UBC 915, UBC 823, UBC 824 and UBC 855 . The marker UBC 815was specific to Panama Tall (PNT) and Saint Vincent Tall (STVT). The marker 1673 UBC 854m was specific to Nuwehnug Tall (NWHT). ISSR markers were proved to be useful for generating fingerprints and for deriving genetic relationships in coconut.en_US
dc.identifier.citationInternational Journal of Innovative Horticulture, Vol.1(1) 2012, p.63-67en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3500
dc.language.isoenen_US
dc.subjectcoconuten_US
dc.subjectISSR markersen_US
dc.subjectgermplasmen_US
dc.subjectspecific markersen_US
dc.titleInter Simple Sequence Repeat (ISSR) markers for identification of germplasm and diversity analysis in coconut (Cocos nucifera L.)en_US
dc.typeArticleen_US

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