Multiple gene analyses reveal extensive genetic diversity among 'Candidatus Phytoplasma mali' populations
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Date
2011
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Abstract
This study focused on evaluating the genetic diversity among 'Candidatus Phytoplasma mali' (‘Ca . P. mali') populations in orchards of north-western Italy, where apple proliferation (AP) disease is widespread and induces severe economic losses. 'Ca. P. mali' was detected through restriction fragment length polymorphism(RFLP) analysis of PCR-amplified 16S rDNA in 101 of 114 samples ,examined. Collective RFLP patterns, obtained by restriction analyses of four amplified genomic segments (16S/23S rDNA. PR-1, PR-2 and PR-3 non ribosomal region, ribosomal protein genes rplV-rpsC and secY gene), revealed the presence of 12 distinct genetic lineages among 60 selected representat ive 'Ca. •P. mali' isolates, underscoring an unexpected high degree of genetic heterogeneity among AP phytoplasma populations in north-western Italy, Prevalence of distinct genetic lineages in diverse geographic regions opens new interesting avenues for studying the epidemiology of AP disease. Furthermore, lineage-specific molecular markers identified in this work could be useful for investigating the biological life cycle of ' Ca. P. mali'
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16S rDNA, apple proliferation, restriction fragment length polymorphism, secY, single nucleotide polymorphism
Citation
Annals of Applied Biology 2011 Vol.158 pp.257-266