Somatic Embryogenesis and Plant Regeneration of Litchi ( Litchi Chinensis Sonn.) From Leaves of Mature Phase Trees
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Date
2007-05
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Publisher
Springer-Verlag
Abstract
Embryogenic cultures were induced from leaflets from new vegetative flushes of mature ‘Brewster’ litchi trees on B5 medium containing 400 mg l -1 glutamine, 200 mg l -1 casein hydrolysate, 30 g l -1 sucrose, 4.52 µM 2,4-D, 9.30 µM kinetin and 3 g l -1 gellan gum in darkness. Embryogenic cultures consisting of proembryonic cells and masses were maintained either on semi-solid MS medium supplemented with 4.52 µM 2,4-D and 0.91 µM zeatin or as embryogenic suspension cultures in liquid medium of the same composition. Maturation of somatic embryos occurred on semi-solid MS medium with 5–20% (v/v) filter-sterilized coconut water in darkness. Recovery of plants from somatic embryos was improved with 14.4 µM GA3 on half-strength MS medium with 0.2 g l -1 activated charcoal under a 16 h photoperiod provided by cool white fluorescent lights (60–80 µmol s -1 m -2 ). Plants have been successfully acclimatized in the greenhouse.
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Keywords
Lychee, Somatic embryo, Suspension culture
Citation
Plant Cell, Tissue and Organ Culture Vol.: 89, No.: 2-3, May 2007 [Page 113-119]