Somatic Embryogenesis and Plant Regeneration of Litchi ( Litchi Chinensis Sonn.) From Leaves of Mature Phase Trees

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dc.contributor.authorSimon H.T. Raharjo
dc.contributor.authorRichard E. Litz
dc.date.accessioned2014-05-19T09:03:09Z
dc.date.available2014-05-19T09:03:09Z
dc.date.issued2007-05
dc.description.abstractEmbryogenic cultures were induced from leaflets from new vegetative flushes of mature ‘Brewster’ litchi trees on B5 medium containing 400 mg l -1 glutamine, 200 mg l -1 casein hydrolysate, 30 g l -1 sucrose, 4.52 µM 2,4-D, 9.30 µM kinetin and 3 g l -1 gellan gum in darkness. Embryogenic cultures consisting of proembryonic cells and masses were maintained either on semi-solid MS medium supplemented with 4.52 µM 2,4-D and 0.91 µM zeatin or as embryogenic suspension cultures in liquid medium of the same composition. Maturation of somatic embryos occurred on semi-solid MS medium with 5–20% (v/v) filter-sterilized coconut water in darkness. Recovery of plants from somatic embryos was improved with 14.4 µM GA3 on half-strength MS medium with 0.2 g l -1 activated charcoal under a 16 h photoperiod provided by cool white fluorescent lights (60–80 µmol s -1 m -2 ). Plants have been successfully acclimatized in the greenhouse.en_US
dc.identifier.citationPlant Cell, Tissue and Organ Culture Vol.: 89, No.: 2-3, May 2007 [Page 113-119]en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1462
dc.language.isoenen_US
dc.publisherSpringer-Verlagen_US
dc.subjectLycheeen_US
dc.subjectSomatic embryoen_US
dc.subjectSuspension cultureen_US
dc.titleSomatic Embryogenesis and Plant Regeneration of Litchi ( Litchi Chinensis Sonn.) From Leaves of Mature Phase Treesen_US
dc.typeArticleen_US

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