Immobilization of Candida antarctica Lipase B by Adsorption to Green Coconut Fiber

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dc.contributor.authorAlvaro D.T. Pinheiro
dc.contributor.authorAna I.S. Brigida
dc.contributor.authorLuciana R.B. Goncalves
dc.contributor.authorAndrea L.O. Ferreira
dc.date.accessioned2014-05-03T10:24:30Z
dc.date.available2014-05-03T10:24:30Z
dc.date.issued2008
dc.description.abstractAn agroindustrial residue, green coconut fiber, was evaluated as support for immobilization of Candida antarctica type B (CALB) lipase by physical adsorption. The influence of several parameters, such as contact time, amount of enzyme offered to immobilization, and pH of lipase solution was analyzed to select a suitable immobilization protocol. Kinetic constants of soluble and immobilized lipases were assayed. Thermal and operational stability of the immobilized enzyme, obtained after 2 h of contact between coconut fiber and enzyme solution, containing 40 U/ml in 25 mM sodium phosphate buffer pH 7, were determined. CALB immobilization by adsorption on coconut fiber promoted an increase in thermal stability at 50 and 60 °C, as half-lives (t1/2) of the immobilized enzyme were, respectively, 2- and 92-fold higher than the ones for soluble enzyme. Furthermore, operational stabilities of methyl butyrate hydrolysis and butyl butyrate synthesis were evaluated. After the third cycle of methyl butyrate hydrolysis, it retained less than 50% of the initial activity, while Novozyme 435 retained more than 70% after the tenth cycle. However, in the synthesis of butyl butyrate, CALB immobilized on coconut fiber showed a good operational stability when compared to Novozyme 435, retaining 80% of its initial activity after the sixth cycle of reaction.en_US
dc.identifier.citationAppl Biochem Biotechnol (2008) 146:173–187en_US
dc.identifier.urihttp://hdl.handle.net/123456789/926
dc.language.isoenen_US
dc.subjectCandida antarctica lipase Ben_US
dc.subjectEnzyme immobilizationen_US
dc.subjectCoconut fiberen_US
dc.subjectPhysical adsorptionen_US
dc.subjectMethyl butyrate hydrolysisen_US
dc.subjectButyl butyrate synthesisen_US
dc.titleImmobilization of Candida antarctica Lipase B by Adsorption to Green Coconut Fiberen_US
dc.typeArticleen_US

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