Diagnosis of baculovirus infection in coconut rhinoceros beetles by examination of excreta

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Date

1986

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Abstract

Oryctes rhinoceros beetles, when infected with Oryctes baculovirus, excreted gut epithelial cells having hypertrophied nuclei, between the third and ninth day post-inoculation. Detection of such abnormal nuclei in the excreta was used as a method of diagnosis of baculovirus infection in field-collected beetles, needed alive for various experiments. This method eliminates the need for sacrificing the beetles for mid-gut examination, a routine method of diagnosis.

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Keywords

Oryctes rhinoceros, baculovirus infection, excretion, epithelial cells, nuclei, hypertrophied, diagnostic method

Citation

Journal of Plant Diseases and Protection, 93(4), 379-383, 1986

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