High-frequency plantlet regeneration and multiple shoot induction from cultured immature seeds of Rhynchostylis retusa Blume., an exquisite orchid

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dc.contributor.authorDennis Thomas, T.
dc.contributor.authorAlwin Michael
dc.date.accessioned2014-05-03T08:43:17Z
dc.date.available2014-05-03T08:43:17Z
dc.date.issued2007
dc.description.abstractSeeds of an exquisite orchid, Rhynchostylis retusa, germinated in vitro on ½ Murashige and Skoog (MS) medium supplemented with different concentrations of coconut milk (CM). Of the different concentrations of CM employed for seed germination, 15% gave optimum response. On this medium a maximum of 93% cultures produced seedlings 90 days after inoculation. Individual seedlings with a length of about 0.5 cm were subcultured on MS medium supplemented with various concentrations of 6-benzylaminopurine (BA) and a-naphthalene acetic acid (NAA), with or without activated charcoal (AC), for further growth. Seedling growth was maximum on MS medium supplemented with 6 lM BA, 0.2 lM NAA, and 1 g L–1 AC. Here a maximum seedling length of 2.3 cm was observed after 1 month of culture. The seedlings were subcultured on MS medium supplemented with kinetin (Kn) or thidiazuron (TDZ), in the presence or absence of AC, for multiple shoot induction. A maximum multiple shoot number of 8.2 was observed on MS medium supplemented with 2 lM TDZ in the presence of AC. The shoots were rooted on ½ MS medium supplemented with 2 lM indole-3-butyric acid (IBA) and successfully transplanted to soil. Of the 45 plantlets transferred to soil 40 survived. The reproducible protocol standardized here will enable rapid propagation and conservation of this precious orchid.en_US
dc.identifier.citationPlant Biotechnol Rep (2007) 1:243–249en_US
dc.identifier.urihttp://hdl.handle.net/123456789/905
dc.language.isoenen_US
dc.subjectOrchidaceaeen_US
dc.subjectRhynchostylis retusaen_US
dc.subjectRegenerationen_US
dc.subjectMultiple shootsen_US
dc.subjectConservationen_US
dc.titleHigh-frequency plantlet regeneration and multiple shoot induction from cultured immature seeds of Rhynchostylis retusa Blume., an exquisite orchiden_US
dc.typeArticleen_US

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