Aflatoxin production by Aspergillus flavus isolates pathogenic to coconut insect pests

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dc.contributor.authorAlka Gupta
dc.contributor.authorMurali Gopal
dc.date.accessioned2014-05-23T06:40:49Z
dc.date.available2014-05-23T06:40:49Z
dc.date.issued2007-02-08
dc.description.abstractAspergillus flavus isolated from naturally infected leaf-eating caterpillar (Opisina arenosella W.), lace bug (Stephanitis typica D.) and plant hopper (Proutista moesta Westwood), insect pests of the coconut palm, were tested for aflatoxin (AT) production by employing various media formulations. These A. flavus isolates were earlier found to be entomopathogenic in laboratory bioassays. A laboratory contaminant and four standard aflatoxigenic A. flavus isolates were also included in this study as reference strains. All A. flavus isolates were tested on seven AT detection media: coconut extract agar, coconut extract-sodium desoxycholate agar, coconut extract-ascorbic acid agar, coconut extract-Czapek Dox agar, coconut extract-milk powder agar, 10% commercial coconut milk powder agar (CCMPA) and 20% CCMPA. Only two isolates of A. flavus, originally isolated from O. arenosella and P. moesta, produced ATs. AT production was detected within 48 h of incubation and was detected continually up to 1 month. These AT-producing A. flavus isolates also produced bright yellow pigmentation in the medium. Of all the seven media used for AT detection, CCMPA (10%) was found to be the best one, followed by 20% CCMPA, for direct and rapid AT detection. AT production was not necessary for pathogenicity in the insects.en_US
dc.identifier.citationWorld journal of microbiology and biotechnology 2002 v-18 p-325-331en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1646
dc.language.isoenen_US
dc.subjectAflatoxinen_US
dc.subjectAspergillus flavusen_US
dc.subjectcoconut insect pestsen_US
dc.subjectentomopathogenicen_US
dc.subjectOpisina arenosella (leaf-eating caterpillar)en_US
dc.subjectProutista moesta (plant hopper)en_US
dc.subjectrapid detectionen_US
dc.subjectStephanitis typica (lace bug)en_US
dc.titleAflatoxin production by Aspergillus flavus isolates pathogenic to coconut insect pestsen_US
dc.typeArticleen_US

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