Response of Oryctes Rhinoceros Larvae to Infection by Oryctes Baculovirus.
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1985
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Three larval instars of Oryctes rhinoceros (L.) were infected with Oryctes baculovirus through contaminated feed. The primary external pathological symptom was translucency of the thorax. Internally, the midgut contained turbid fluid with flakes of sloughed-off epithelial tissue. Baculovirus infection in larvae could be diagnosed in Giemsa stained scrappings of the midgut epithelium, two days after inoculation, whereas bioassay test could detect infection on the third day. Median lethal dose (LD50) and weight of infected tissue containing one LD50 dose for all the three instars were computed. The second instar was the least susceptible to virus infection. The order of susceptibility among the instars was 1>III>II. Translucency was found to appear 3-4 days prior to death. Lethal infection period ranged from eleven to sixteen days. The thermal inactivation point of the virus in tissue suspension was at 56C.
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J.Plantn.Crops 1985 v-13 i-2 p-116-124