Capsicum chinense Jacq.‑derived glutaredoxin (CcGRXS12) alters redox status of the cells to confer resistance against pepper mild mottle virus (PMMoV‑I)

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dc.contributor.authorR. M. Saravana Kumar
dc.contributor.authorRamesh, S.V.
dc.contributor.authorSugitha Thankappan
dc.contributor.authorNaga Prafulla Chandrika Nulu
dc.contributor.authorAsish Kanakaraj Binodh
dc.contributor.authorSundaravelpandian Kalaipandian
dc.contributor.authorRamachandran Srinivasan
dc.date.accessioned2025-12-05T05:40:26Z
dc.date.available2025-12-05T05:40:26Z
dc.date.issued2024
dc.description.abstractGlutaredoxins (Grxs) are small, ubiquitous and multi-functional proteins. They are present in different compartments of plant cells. A chloroplast targeted Class I GRX (CcGRXS12) gene was isolated from Capsicum chinense during the pepper mild mottle virus (PMMoV) infection. Functional characterization of the gene was performed in Nicotiana benthamiana transgenic plants transformed with native C. chinense GRX (Nb:GRX), GRX-fused with GFP (Nb:GRX-GFP) and GRX-truncated for chloroplast sequences fused with GFP (Nb:Δ2MGRX-GFP). Overexpression of CcGRXS12 inhibited the PMMoV-I accumulation at the later stage of infection, accompanied with the activation of salicylic acid (SA) pathway pathogenesis-related (PR) transcripts and suppression of JA/ET pathway transcripts. Further, the reduced accumulation of auxin-induced Glutathione-S-Transferase (pCNT103) in CcGRXS12 overexpressing lines indicated that the protein could protect the plants from the oxidative stress caused by the virus. PMMoV-I infection increased the accumulation of pyridine nucleotides (PNs) mainly due to the reduced form of PNs (NAD(P)H), and it was high in Nb:GRX-GFP lines compared to other transgenic lines. Apart from biotic stress, CcGRXS12 protects the plants from abiotic stress conditions caused by H2O2 and herbicide paraquat. CcGRXS12 exhibited GSH-disulphide oxidoreductase activity in vitro; however, it was devoid of complementary Fe–S cluster assembly mechanism found in yeast. Overall, this study proves that CcGRXS12 plays a crucial role during biotic and abiotic stress in plants.
dc.identifier.citationPlant Cell Reports (2024) 43:108 https://doi.org/10.1007/s00299-024-03174-2
dc.identifier.urihttp://14.139.158.120:4000/handle/123456789/8893
dc.language.isoen
dc.subjectPepper mild mottle virus
dc.subjectItalian strain (PMMoV-I)
dc.subjectGlutaredoxin (GRX)
dc.subjectPyridine nucleotides (PNs)
dc.subjectGlutathione-S
dc.subjectTransferase (GST)
dc.subjectGlutathione (GSH)
dc.subjectPathogenesis related (PR)
dc.titleCapsicum chinense Jacq.‑derived glutaredoxin (CcGRXS12) alters redox status of the cells to confer resistance against pepper mild mottle virus (PMMoV‑I)
dc.typeArticle

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