Biochemical evidences for purification of phytoplasma of root (wilt) disease of coconut
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Date
2007-02-08
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Abstract
Phytoplasma of root (wilt) disease of coconut (Cocos nucifera L.) has been purified from tender leaf tissues of diseased palms by Percoll-discontinuous density gradient centrifugation (PDDGC). Purified phytoplasma fractions were checked by biochemical methods such as ultraviolet-absorption spectrum (190-310 nm) and sodium dodecyl sulphate-polyacrylamide gel elcctrophoresis (SDS-PAGE). PDDGC produced a single turbid zone of phytoplasma at the top of 30% step gradient from diseased leaf samples, which was not seen in samples from healthy leaf. Phytoplasma from this fraction had an absorption maximum near 200 nm. On further fractionation, the protein and nucleic acid components of phytoplasma showed absorption peaks at 280 and 260 nm respectively. The protein of phyloplasma from diseased leaf samples produced three major peptides of 29, 28 and 18.5 kDa in SDS-PAGE.
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Coconut, root (wilt) disease, phytoplasma, purification
Citation
Journal plant biology 2001 v-28 i-1 p-55-60