Isolation and Characterization of Mutants of the Plant Growth-Promoting Rhizobacterium Pseudomonas putida GR12-2 That Overproduce Indoleacetic Acid
| dc.contributor.author | Hong Xie | |
| dc.contributor.author | Pasternak, J.J. | |
| dc.contributor.author | Bernard R. Glick | |
| dc.date.accessioned | 2014-09-02T10:56:56Z | |
| dc.date.available | 2014-09-02T10:56:56Z | |
| dc.date.issued | 1996 | |
| dc.description.abstract | Following transposon Tn5 mutagenesis of the plant growth-promoting rhizobacteTium Pseudomonas putida GR12-2, mutants that were able to grow in the presence of the tryptophan analog 5-fluorotryptophan were selected. Seven of the 50 5-fluorotryptophan-resistant mutants overproduced the phytohormone indoleacetic acid (IAA). Of these seven mutants, the highest level of IAA was observed with strain P. putida GR12-2/auxl,which produced four times the amount of indoleacetic acid synthesized by the wild-type strain. Strain P. putida GR12-2/auxl, in contrast to the wild type, lost the ability to stimulate the elongation of the roots of canola seedlings under gnotobiotic conditions. The growth rate, siderophore production, and 1-aminocyclopropane-1-carboxylate deaminase activity of mutant strain P. putida GR12-2/auxl were identical to those of the wild-type strain. The role of IAA in the mechanism of plant growth stimulation by P. putida GR12-2 and other plant growth-promoting rhizobacteria is discussed. | en_US |
| dc.identifier.citation | Current Microbiology Vol.32(1996) pp.67-71 | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/5294 | |
| dc.language.iso | en | en_US |
| dc.title | Isolation and Characterization of Mutants of the Plant Growth-Promoting Rhizobacterium Pseudomonas putida GR12-2 That Overproduce Indoleacetic Acid | en_US |
| dc.type | Article | en_US |