Optimization of DNA amplification fingerprinting parameters in coconut (Cocos nucifera L.)

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dc.contributor.authorManimekalai, R.
dc.contributor.authorJeya Dev, K.
dc.contributor.authorAnuradha Upadhyay
dc.contributor.authorDevakumar, K.
dc.contributor.authorRajesh, M.K.
dc.contributor.authorParthasarathy, V.A.
dc.contributor.authorRajagopal, V.
dc.contributor.authorKumaran, P.M.
dc.date.accessioned2014-07-10T06:32:45Z
dc.date.available2014-07-10T06:32:45Z
dc.date.issued2004-12
dc.description.abstractDNA Amplification Fingerprinting (DAF) is a simple and powerful technique for genome analysis. It is being employed in various applications in a number of crops. For obtaining reproducible results from DAF technique, optimization of PCR parameters was done. 20 ng (2 ng/ µl) template DNA, 15 µM primer, 250 µM each of dNTPs, 2 U (0.2 U/µl) Taq polymerase, 4 mM MgCl2, and annealing temperature of 55 "C, with standard silver staining protocol gave good amplification with reproducibility.S ubsequently these results will be used in tagging resistance genes for root (wilt) disease in coconut. The effect of GC content of primers was also discussed.en_US
dc.identifier.citationJournal of Plantation Crops 32(3) : 1-5en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3968
dc.language.isoenen_US
dc.subjectCoconuten_US
dc.subjectDNA amplification fingerprinting (DAF)en_US
dc.subjectoptimizationen_US
dc.titleOptimization of DNA amplification fingerprinting parameters in coconut (Cocos nucifera L.)en_US
dc.typeArticleen_US

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