An efficient method of DNA extraction from coconut leaves
Loading...
Date
2007-02-08
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A protocol for extraction of good quality, high molecular weight genomic DNA from young leaves of coconut was standardized using two detergents viz- sodium dodecyl sulphate (SDS) and hexadecyl trimethyl ammonium bromide (CTAB) at different concentrations; at pH 8.0 and pH 9.0. SDS (1%) at pH 8.0 was found to yield good quantity of high quality DNA. The extracted DNA was amenable to PCR and restriction digestion.
Description
Keywords
Coconut, DNA, protocol, PCR, RFLP
Citation
Agrotropica 1999 v-11 i-1 p-35-38