An efficient method of DNA extraction from coconut leaves
dc.contributor.author | Anuradha Upadhyay | |
dc.contributor.author | Parthasarathy, V.A. | |
dc.contributor.author | Seema, G. | |
dc.contributor.author | Anitha Karun | |
dc.date.accessioned | 2014-06-12T10:27:23Z | |
dc.date.available | 2014-06-12T10:27:23Z | |
dc.date.issued | 2007-02-08 | |
dc.description.abstract | A protocol for extraction of good quality, high molecular weight genomic DNA from young leaves of coconut was standardized using two detergents viz- sodium dodecyl sulphate (SDS) and hexadecyl trimethyl ammonium bromide (CTAB) at different concentrations; at pH 8.0 and pH 9.0. SDS (1%) at pH 8.0 was found to yield good quantity of high quality DNA. The extracted DNA was amenable to PCR and restriction digestion. | en_US |
dc.identifier.citation | Agrotropica 1999 v-11 i-1 p-35-38 | en_US |
dc.identifier.uri | http://hdl.handle.net/123456789/2870 | |
dc.language.iso | en | en_US |
dc.subject | Coconut | en_US |
dc.subject | DNA | en_US |
dc.subject | protocol | en_US |
dc.subject | PCR | en_US |
dc.subject | RFLP | en_US |
dc.title | An efficient method of DNA extraction from coconut leaves | en_US |
dc.type | Article | en_US |
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