Evaluation of molecular diversity of ex situ conserved germplasm of palmyrah (Borassus flabellifer L.) accessions using RAPD markers
No Thumbnail Available
Date
2016-08
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The genetic relationship of 96 palmyrah palms, consisting of 24 indigenous accessions from Tamil Nadu and Andhra Pradesh,
was investigated using Random Amplified Polymorphic DNA (RAPD) markers. Hundred and eighty primers were used initially
to identify the polymorphic primers in six random samples and 10 polymorphic primers were selected to amplify the 96 palms.
These 10 primers produced a total of 112 reproducible bands and out of them, 41 fragments (36.6 %) showed polymorphism. The
number of bands produced with each primer varied from seven to 15 with an average of 11.2 bands per primer. The percent
polymorphism ranged from 7.7 to 71.4 with an average of 37.4 per cent when all the primers were taken collectively. UPGMA
grouped all the accessions into two major clusters at 0.85 similarity value. The highest similarity value (0.96) was observed
between the accessions KLKM-8 and THY-54 and the lowest similarity value (0.782) was obtained between ANBI-17 and
RCML-11. The relatively low polymorphism suggests a narrow genetic diversity of palmyrah populations from which the present
accessions have been derived and maintained over the years.
Description
Keywords
Borassus flabellifer, genetic diversity, germplasm, RAPD
Citation
Journal of Plantation Crops, 2016, 44(2): 96-102