dsRNA-induced gene silencing in Moniliophthora perniciosa, the causal agent of witches’ broom disease of cacao

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dc.contributor.authorCaribe dos Santos, A.C.
dc.contributor.authorSena, J.A.L.
dc.contributor.authorSantos, S.C.
dc.contributor.authorDias, C.V.
dc.contributor.authorPirovani, C.P.
dc.contributor.authorPungartnik, C.
dc.contributor.authorValle, R.R.
dc.contributor.authorCascardo, J.C.M.
dc.contributor.authorVincentz, M.
dc.date.accessioned2014-04-07T08:43:22Z
dc.date.available2014-04-07T08:43:22Z
dc.date.issued2009
dc.description.abstractThe genome sequence of the hemibiotrophic fungus Moniliophthora perniciosa revealed genes possibly participating in the RNAi machinery. Therefore, studies were performed in order to investigate the efficiency of gene silencing by dsRNA. We showed that the reporter gfp gene stably introduced into the fungus genome can be silenced by transfection of in vitro synthesized gfpdsRNA. In addition, successful dsRNA-induced silencing of endogenous genes coding for hydrophobins and a peroxiredoxin were also achieved. All genes showed a silencing efficiency ranging from 18% to 98% when compared to controls even 28 d after dsRNA treatment, suggesting systemic silencing. Reduction of GFP fluorescence, peroxidase activity levels and survival responses to H2O2 were consistent with the reduction of GFP and peroxidase mRNA levels, respectively. dsRNA transformation of M. perniciosa is shown here to efficiently promote genetic knockdown and can thus be used to assess gene function in this pathogen.en_US
dc.identifier.citationFungal Genetics and Biology 46 (2009) 825–836en_US
dc.identifier.urihttp://hdl.handle.net/123456789/281
dc.language.isoenen_US
dc.subjectCrinipellis perniciosaen_US
dc.subjectTheobroma cacaoen_US
dc.subjectRNAien_US
dc.subjectPeroxidaseen_US
dc.subjectGFPen_US
dc.subjectHydrophobinsen_US
dc.titledsRNA-induced gene silencing in Moniliophthora perniciosa, the causal agent of witches’ broom disease of cacaoen_US
dc.typeArticleen_US

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