Influence of freezable/non-freezable water and sucrose on the viability of Theobroma cacao somatic embryos following desiccation and freezing

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dc.contributor.authorJong-Yi Fang
dc.contributor.authorMoctar Sacande
dc.contributor.authorHugh Pritchard
dc.contributor.authorAndy Wetten
dc.date.accessioned2014-04-11T06:07:43Z
dc.date.available2014-04-11T06:07:43Z
dc.date.issued2009
dc.description.abstractEncapsulated cocoa (Theobroma cacao L.) somatic embryos subjected to 0.08–1.25 M sucrose treatments were analyzed for embryo soluble sugar content, nonfreezable water content, moisture level after desiccation and viability after desiccation and freezing. Results indicated that the higher the sucrose concentration in the treatment medium, the greater was the extent of sucrose accumulation in the embryos. Sucrose treatment greatly assisted embryo post-desiccation recovery since only 40% of the control embryos survived desiccation, whereas a survival rate of 60–95% was recorded for embryos exposed to 0.5–1.25 M sucrose. The non-freezable water content of the embryos was estimated at between 0.26 and 0.61 g H2O g-1dw depending on the sucrose treatment, and no obvious relationship could be found between the endogenous sucrose level and the amount of non-freezable water in the embryos. Cocoa somatic embryos could withstand the loss of a fraction of their non-freezable water without losing viability following desiccation. Nevertheless, the complete removal of potentially freezable water was not sufficient for most embryos to survive freezing.en_US
dc.identifier.citationPlant Cell Rep (2009) 28:883–889en_US
dc.identifier.urihttp://hdl.handle.net/123456789/352
dc.language.isoenen_US
dc.subjectFreezable wateren_US
dc.subjectNon-freezable wateren_US
dc.subjectDesiccationen_US
dc.subjectFreezingen_US
dc.subjectSucroseen_US
dc.subjectCocoaen_US
dc.subjectTheobroma cacaoen_US
dc.subjectSomatic embryoen_US
dc.subjectCryopreservationen_US
dc.titleInfluence of freezable/non-freezable water and sucrose on the viability of Theobroma cacao somatic embryos following desiccation and freezingen_US
dc.typeArticleen_US

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