DNA polymorphism among coconut (Cocos nucifera L.) cultivars and reciprocal cross derivatives differing in drought tolerance
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Date
2004-12
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Abstract
Coconut seedlings of Laccadive Ordinary Tall (LCT). Gangabondam Green Dwarf (GBGD) and their reciprocal cross combinations were analysed by molecular markers viz. Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR). These seedlings were evaluated for water stress tolerance based on leaf water potential measured during stress and non- stress periods. Thc leaf water potential decreased during stress period. The differences among the individuals for leaf water poteniial during stress were not significant. The molecular markers revealed the genetic structure of the cultivars and derivatives clearly. In the dendrogram. individuals of GBGD and GBGD X LCT formed one cluster and LCT and LCT X GBGD formed another cluster. The individuals of LCT and LCT X GBGD showed variation among themselves and they were scattered in the principal co-ordinate plot. Even though the parents are same. the reciprocal cross derivatives behaved differently for leaf water potential and showed polymorphism at DNA level. When the dwarf palm was used as female parent, the F, s were similar to female parent in their banding pattern.
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Keywords
Coconut, DNA, RAPD, ISSR, drought tolerance
Citation
Journal of Plantation Crops 32(Suppl.) December 2004: 117-122