Isolation, cDNA cloning and gene expression of an antibacterial protein from larvae of the coconut rhinoceros beetle, Oryctes rhinoceros

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dc.contributor.authorJun YANG
dc.contributor.authorJun ISHIBASHI
dc.contributor.authorMasanori YAMAMOTO
dc.contributor.authorKiyoko TANIAI
dc.contributor.authorMinoru YAMAKAWA
dc.date.accessioned2014-05-07T08:36:06Z
dc.date.available2014-05-07T08:36:06Z
dc.date.issued1998
dc.description.abstractAn antibacterial protein, designated rhinocerosin, was purified to homogeneity from larvae of the coconut rhinoceros beetle, Oryctes rhinoceros immunized with Escherichia coli. Based on the amino acid sequence of the N-terminal region, a degenerate primer was synthesized and reverse-transcriptase PCR was performed to clone rhinocerosin cDNA. As a result, a 279-bp fragment was obtained. The complete nucleotide sequence was determined by sequencing the extended rhinocerosin cDNA clone by 5ยข rapid amplification of cDNA ends. The deduced amino acid sequence of the mature portion of rhinocerosin was composed of 72 amino acids without cystein residues and was shown to be rich in glycine (11.1%) and proline (11.1%) residues. Comparison of the deduced amino acid sequence of rhinocerosin with those of other antibacterial proteins indicated that it has 77.8% and 44.6% identity with holotricin 2 and coleoptrecin, respectively. Rhinocerosin had strong antibacterial activity against E. coli, Streptococcus pyogenes, Staphylococcus aureus but not against Pseudomonas aeruginosa. Results of reverse-transcriptase PCR analysis of gene expression in different tissues indicated that the rhinocerosin gene is strongly expressed in the fat body and the Malpighian tubule, and weakly expressed in hemocytes and midgut. In addition, gene expression was inducible by bacteria in the fat body, the Malpighian tubule and hemocyte but constitutive expression was observed in the midgut.en_US
dc.identifier.citationEur. J. Biochem. 255, 7342738 (1998)en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1028
dc.language.isoenen_US
dc.subjectantibacterial proteinen_US
dc.subjectOryctes rhinocerosen_US
dc.subjectinsect immunityen_US
dc.subjectcDNA cloningen_US
dc.subjectgene expressionen_US
dc.titleIsolation, cDNA cloning and gene expression of an antibacterial protein from larvae of the coconut rhinoceros beetle, Oryctes rhinocerosen_US
dc.typeArticleen_US

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