Micropropagation of an endangered Indian sandalwood (Santalum album L.)
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Date
2006
Journal Title
Journal ISSN
Volume Title
Publisher
The Japanese Forest Society and Springer-Verlag
Abstract
Santalum album is known as East Indian sandalwood. It is the most economically important tree harvested for heartwood oil, and India is among the chief exporters of sandalwood and its products. Multiple shoots were induced from nodal shoot segments derived from a 50- to 60-year-old candidate plus tree (CPT) on Murashige and Skoog (MS) medium supplemented with 0.53mM a-naphthaleneacetic acid (NAA) and 11.09mM 6-benzylaminopurine (BA). In vitro differentiated shoots were multiplied on MS medium with 0.53mM NAA, 4.44mM BA, and additives: 283.93mM ascorbic acid, 118.10mM citric acid, 104.04mM cystine, 342.24mM glutamine, and 10% (v/v) coconut milk. New shoots were harvested repeatedly for up to three subculture passages on fresh medium at 4-week intervals. Microshoots treated with 98.4mM indole-3-butyric acid (IBA) for 48 h produced roots on growth-regulator-free, quarter-strength MS basal salts medium with vitamin B5 and 2% sucrose. In vitro root induction was achieved from microshoots pulsed with 1230mM IBA for 30min in soilrite rooting medium. The percentage of rooting in soilrite was higher than that for agar medium, and in vitro raised plants were established in the field and showed normal growth.
Description
Keywords
Chandan, Tissue culture, Mature tree, Axillary shoot proliferation, In vitro and ex vitro rooting
Citation
J For Res (2006) 11:203–209