In vitro flowering of green and albino Dendrocalamus latiflorus

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dc.contributor.authorChoun-Sea Lin
dc.contributor.authorHsaio, H.W.
dc.contributor.authorLiang, C.J.
dc.contributor.authorLin, M.J.
dc.contributor.authorChang, W.C.
dc.date.accessioned2014-05-07T06:21:51Z
dc.date.available2014-05-07T06:21:51Z
dc.date.issued2007
dc.description.abstractTo propagate Dendrocalamus latiflorus, we used in vivo inflorescences to produce calli on Murashige and Skoog basal (MS) medium supplemented with 3 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 2 mg/l kinetin, 250 mg/l polyvinyl pyrrolidone (PVP), and 1% coconut milk. Multiple shoots were generated on MS medium supplemented with 0.1 mg/l thidiazuron (TDZ). The green plantlets were successfully transferred to soil. Multiple albino shoots also regenerated and were able to proliferate on medium containing cytokinins, especially TDZ. Albino multiple shoots rooted in medium containing a-naphthaleneacetic acid (NAA), and callus formation was observed in the presence of 2,4-D and picloram. Green and albino regenerates flowered after 8 months of subculture. The flowering ratio increased to 44% after three treatments in medium containing 1 mg/l TDZ. Morphological observations revealed that the in vitro green and albino flower organs were normal. However, pollen derived from the in vitro flowers of both the green and albino plants were sterile.en_US
dc.identifier.citationNew Forests (2007) 34:177–186en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1012
dc.language.isoenen_US
dc.subjectBambooen_US
dc.subjectCytokininen_US
dc.subjectTDZen_US
dc.titleIn vitro flowering of green and albino Dendrocalamus latiflorusen_US
dc.typeArticleen_US

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