In vitro maturation and germination of Orychophragmus violaceus microspores

Simple item page
dc.contributor.authorYaodong Li
dc.contributor.authorXiaoguang Zhao
dc.contributor.authorWenguo Wang
dc.contributor.authorShenghua Wang
dc.contributor.authorFang Chen
dc.contributor.authorJie Xing
dc.date.accessioned2014-05-07T06:41:23Z
dc.date.available2014-05-07T06:41:23Z
dc.date.issued2007
dc.description.abstractMicrospores cultured in vitro can be regarded as a system to study gene regulation, cell fate determination and cell differentiation during pollen development as well as an alternative method of genetic transformation in plants. In our study, pollen development and viability in Orychophragmus violaceus in vivo were determined and then pollen from the late unicellular stage was cultured in vitro. MS liquid medium + White vitamins + 2% (V/V) coconut milk + 0.5 M maltose, pH = 7.0 was the most appropriate for in vitro culture of Orychophragmus violaceus microspores. With this medium, the rates of in maturation and germination were 19.3% and 4.7%, respectively. Liquid medium with 0.6 M maltose + 1.6 mM boric acid + 2.9 mM Ca(NO3)2 + 29.6 lM vitamin B1, pH = 7.0 was optimal for germination of pollen matured in vivo. The rate of germination was 70.7%. Pollen matured in vitro cultured in similar medium exhibited a rate of germination of 62.7%. Hence, the experimental study showed that in vitro maturation of microspores is feasible and this experimental system can be applied to further theoretical and practical research.en_US
dc.identifier.citationPlant Cell Tiss Organ Cult (2007) 91:53–60en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1017
dc.language.isoenen_US
dc.subjectBrassicaceaeen_US
dc.subjectPollen germinationen_US
dc.subjectPollen cultureen_US
dc.subjectViabilityen_US
dc.titleIn vitro maturation and germination of Orychophragmus violaceus microsporesen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
In vitro maturation and germination of Orychophragmus violaceus microspores.pdf
Size:
253.24 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Coconut