Kinetoplast DNA minicircles of phloem-restricted Phytomonas associated with wilt diseases of coconut and oil palms have a two-domain structure
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Date
2001
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Science
Abstract
We report the cloning and sequencing of the first minicircle from a phloem-restricted, pathogenic Phytomonas sp. (Hart 1) isolated from a coconut palm with hartrot disease. The minicircle possessed a two domain structure of two conserved regions, each containing three conserved sequence blocks (CSB). Based on the sequence around CSB 3 from Hart I, PCR primers were designed to allow specific amplification of Phytomonas minicircles. This primer pair demonstrated specificity for at least six groups of plant trypanosomatids and did not amplify from insect trypanosomatids. The PCR results were consistent with a two-domain structure for other plant trypanosomatids.
Description
Keywords
Conserved sequence block, Hartrot, Marchitez sorpressiva, Polymerase chain reaction, Plant trypanosomatid
Citation
FEMS Microbiology Letters 205 (2001) 65-69